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Exalpha 凋亡增殖、炎癥、免疫學(xué)傳染病學(xué)和細(xì)胞間結(jié)構(gòu)研究-相關(guān)技術(shù)文章

更新時(shí)間：2024-07-18

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Exalpha 凋亡增殖、炎癥、免疫學(xué)傳染病學(xué)和細(xì)胞間結(jié)構(gòu)研究

Exalpha公司簡(jiǎn)介

Exalpha有限公司于2000年在馬薩諸塞州雪莉（美國(guó)）成立。該公司主要通過(guò)開(kāi)發(fā)和生產(chǎn)抗體來(lái)支持生命科學(xué)領(lǐng)域的研究人員和科學(xué)家。

Exalpha公司產(chǎn)品領(lǐng)域

Exalpha公司其領(lǐng)域涉及信號(hào)通路、凋亡、增殖、炎癥、免疫學(xué)、傳染病學(xué)和細(xì)胞間結(jié)構(gòu)研究等方面。Exalpha主要產(chǎn)品線(xiàn)包括Proliferation&apoptosis, Enzymes&Proteins, Clinical IHC Markers , GPCRs等多個(gè)產(chǎn)品線(xiàn)，可提供給全球的科研實(shí)驗(yàn)室，制藥企業(yè)，生物技術(shù)和診斷公司，醫(yī)院等，目前旗下的代理公司遍布全球。

細(xì)胞增殖是生物體的重要生命特征，是生物體生長(zhǎng)、發(fā)育、繁殖以及遺傳的基礎(chǔ)。監(jiān)測(cè)細(xì)胞群的生長(zhǎng)對(duì)細(xì)胞的狀態(tài)研究很有必要?？蒲泄ぷ髡邆兘?jīng)常需要檢測(cè)細(xì)胞增殖情況，比如驗(yàn)證抗腫瘤藥物的藥效時(shí)，監(jiān)測(cè)細(xì)胞增殖及存活情況；研究腫瘤侵襲與轉(zhuǎn)移模型時(shí)，分析細(xì)胞轉(zhuǎn)移或侵襲的路徑；分析創(chuàng)傷的修復(fù)或組織的重建時(shí)，檢測(cè)細(xì)胞生長(zhǎng)情況等等。檢測(cè)細(xì)胞增殖及活性變化則可以很好的反映細(xì)胞的生長(zhǎng)狀況。

細(xì)胞增殖的研究方法

細(xì)胞增殖的研究方法有很多種，根據(jù)檢測(cè)原理不同，主要分為DNA合成檢測(cè)法、代謝活性檢測(cè)法、細(xì)胞增殖標(biāo)志物檢測(cè)法等。本文重點(diǎn)介紹DNA合成檢測(cè)之Brdu檢測(cè)法，傳統(tǒng)DNA合成檢測(cè)方法使用胸腺嘧啶核苷（3H-TdR）摻入DNA，然后通過(guò)放射自顯影或閃爍計(jì)數(shù)對(duì)DNA進(jìn)行定量，該方法由于存在放射性物質(zhì)、實(shí)驗(yàn)周期長(zhǎng)、操作繁瑣，且需要昂貴的設(shè)備，目前已被BrdU（5-Bromo-2-deoxyuridine，5-溴脫氧尿嘧啶核苷）檢測(cè)方法取代，BrdU作為一種胸苷類(lèi)似物，可選擇性地?fù)饺朐鲋臣?xì)胞的DNA中，而且可穩(wěn)定存在，并帶到子代細(xì)胞中，細(xì)胞經(jīng)過(guò)固定和變性處理后，可用免疫學(xué)方法檢測(cè)DNA中BrdU的含量，從而判斷細(xì)胞的增殖能力。

Exalpha Biologicals品牌BrdU檢測(cè)試劑盒

因組織細(xì)胞內(nèi)無(wú)內(nèi)源性BrdU存在，可利用BrdU相關(guān)抗體來(lái)檢測(cè)和定位摻入活躍增殖細(xì)胞新合成DNA的BrdU，Exalpha Biologicals 品牌BrdU檢測(cè)試劑盒的優(yōu)勢(shì)在于使用細(xì)胞層作為固相，將BrdU試劑摻入微孔板中的細(xì)胞，除了對(duì)細(xì)胞增殖情況進(jìn)行評(píng)估外，還可以從單個(gè)培養(yǎng)物中分析細(xì)胞數(shù)量、形態(tài)和細(xì)胞抗原等信息。同時(shí)Exalpha Biologicals 品牌BrdU檢測(cè)試劑盒可通過(guò)試劑盒自帶的固定/變性溶液一步完成細(xì)胞固定、透化和DNA變性等實(shí)驗(yàn)流程，大大縮短了實(shí)驗(yàn)時(shí)間。Exalpha Biologicals 品牌BrdU檢測(cè)試劑盒中包含檢測(cè)細(xì)胞增殖活性所需的所有組分、操作簡(jiǎn)便、無(wú)放射性、廣泛的樣本適用性，已得到眾多科研工作者的青睞。

Exalpha公司熱銷(xiāo)產(chǎn)品

產(chǎn)品名稱(chēng)	貨號(hào)	規(guī)格
BrdU Cell Proliferation Assay Kit	X1327K1	200Tests
chx 10 (Visual system homeobox 2) (NT) Polyclonal Antibody	X1180P	250ug
EBNA 3C Polyclonal Antibody	F125P	250ug
SURF1 Polyclonal Antibody	X1562P	100ug
Bnip3L (IN) (nix) (bcl 2 family) Polyclonal Antibody	X1120P	100ug
chx 10 (Visual system homeobox 2) (CT) Polyclonal Antibody	X1179P	250ug
Microphthalmia Transcription Factor (MiTF) Monoclonal Antibody	X1405M	100ug
EBNA 3A Polyclonal Antibody	F115P	250ug
CD5 PE Single-Color Reagent	0054	100Tests
PTP mu Monoclonal Antibody	P100M	100ug
CD117 FITC Single-Color Reagent	1172	100Tests
Kv1.5 Potassium Channel Polyclonal Antibody	X1501P	100ug
N-SMase Activation Associated Factor Antigen Immunoaffinity Purified Polyclonal	X2354P	100ug
Chicken anti Glutathione S Transferase (GST), conjugated with HRP	AGST-HRP	0.1 mg
CD7 PE Single-Color Reagent	0074	100Tests
Protein Phosphatase 1 alpha Polyclonal Antibody	P105P	100ug
CD2 PE Single-Color Reagent	0024	100Tests
Kv1.6Potassium Channel Polyclonal Antibody	X1502P	100ug
Degenerative Spermatocyte Homolog 2 (DEGS2) Antigen Immunoaffinity Purified Polyclonal	X2356P	100ug
CD8 PE Single-Color Reagent	0084	100Tests
Protein Phosphatase 1 beta Polyclonal Antibody	P110P	100ug
CD2 FITC - CD7 PE Bi-Test Reagents (FITC/RPE)	0027S	50Tests
Kv2.1Potassium Channel Polyclonal Antibody	X1503p	100ug
Sphingomyelin Phosphodiesterase 3 Antigen Immunoaffinity Purified Polyclonal	X2359P	100ug
Chicken anti HA (Hemagglutinin) Epitope	AHA	0.1 mg
CD13 PE Single-Color Reagent	0134	100Tests
Protein Phosphatase 1 beta Polyclonal Antibody	P115P	100ug
CD3 PE Single-Color Reagent	0034	100Tests
Potassium Channel (Kv3.1b ) Polyclonal Antibody	X1504P	100ug
Monocarboxylate Transporter 3 (MCT3) Antigen Immunoaffinity Purified Polyclonal	X2361P	100ug
Protein Phosphatase 1 gamma 1 Polyclonal Antibody	P120P	100ug
CD61 PE Single-Color Reagent	0614	100Tests
Potassium Channel (Kv4.2 ) Polyclonal Antibody	X1505P	100ug
Zinc Finger Protein GLI1 Antigen Immunoaffinity Purified Polyclonal	X2363P	100ug
Chicken anti HA (Hemagglutinin) Epitope, conjugated with FITC	AHA-F	0.1 mg
CD16 PE Single-Color Reagent	0164	100Tests
Protein Phosphatase 1 gamma 1 Polyclonal Antibody	P125P	100ug
BrdU (bromodeoxyuridine) Monoclonal Antibody	X1028	100ug
Calcium Channel (a1B) Polyclonal Antibody	X1507P	100ug
Monocarboxylate Transport 2 Antigen Immunoaffinity Purified Polyclonal	X2368P	100ug
Chicken anti HA (Hemagglutinin) Epitope, conjugated with HRP	AHA-HRP	0.1 mg
CD18 PE Single-Color Reagent	0184	100Tests
Protein Phosphatase 1 gamma 2 Polyclonal Antibody	P130P	100ug
Muc1 Monoclonal Antibody	X2783M	100ug
Voltage-dependent T-type calcium channel subunit alpha-1E Polyclonal Antibody	X1509P	100ug
G-Protein Coupled Receptor 94 Antigen Immunoaffinity Purified Polyclonal	X2373P	100ug
CD19 PE Single-Color Reagent	0194	100Tests
Protein Phosphatase 2 A/A Polyclonal Antibody	P140P	100ug
CD95 Monoclonal Antibody	X2785M	100ug
Calcium Channel (a1G) Polyclonal Antibody	X1510P	100ug

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Exalpha 凋亡增殖、炎癥、免疫學(xué)傳染病學(xué)和細(xì)胞間結(jié)構(gòu)研究-相關(guān)技術(shù)文章

Exalpha 凋亡增殖、炎癥、免疫學(xué)傳染病學(xué)和細(xì)胞間結(jié)構(gòu)研究-相關(guān)技術(shù)文章